Freezing induces biased results in the molecular detection of Flavobacterium columnare.

نویسندگان

  • Lotta-Riina Suomalainen
  • Hilkka Reunanen
  • Ritva Ijäs
  • E Tellervo Valtonen
  • Marja Tiirola
چکیده

Specific PCR detection and electron microscopy of Flavobacterium columnare revealed the risk of false-negative results in molecular detection of this fish pathogen. Freezing and thawing destroyed the cells so that DNA was for the most part undetectable by PCR. The detection of bacteria was also weakened after prolonged enrichment cultivation of samples from infected fish.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 72 2  شماره 

صفحات  -

تاریخ انتشار 2006